Technické údaje pro cell^tool TIRFM |
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 Pozorování povrchu buňky bez
rozmazání světlem mimo rovinu
zaostření
Plně integrováno do zobrazovacích
stanic cell^M a ^R
Kombinace až tří laserů
a osvětlovacího systému MT10/20
Optimalizované uspořádání paprsků
v portech jednotlivých laserů |
 Zpět na hlavní stránku |
 Vytisknout |
Multi-port Illuminator
| Multi-port Illuminator |
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Modular design for the combination of up to three lasers plus a MT10/MT20 fluorescence illumination system |
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Individual ports for each laser fibre guarantee optimised beam alignment |
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Field stop for each laser port to reduce the field of illumination |
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Fast shutters (1ms) allow fast switch between all lasers and MT10/MT20 |
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Easy experiment set-up by integration into the Experiment Manager of cell^M and cell^R |
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Including all safety features required by laser safety regulations |
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Class 1 laser product (normal operation), Class 3B (maintenance mode), IEC60825:1 |
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Available for IX2 microscopes |
TIRFM Objectives
| TIRFM objectives |
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PLAPON 60xO (NA 1.42)
UIS2 objective designed for standard fluorescence applications, due to its unusually high NA, it still has the capacity for TIRFM experiments. No compensation collar. |
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PLAPON 60xO TIRFM (NA 1.45)
UIS2 TIRFM objective designed for usage with conventional immersion oil and cover glasses, contains a compensation
collar for temperature and cover glass thickness. |
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PLAPO 100xO TIRFM (NA 1.45)
Excellent high magnification objective for standard immersion oil guarantees high resolution TIRFM images. |
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APO 100xOHR (NA 1.65)
High magnification TIRFM objective, world record holder (Sep. 2005) in light efficiency and resolution with an unparalleled numerical aperture of 1.65. It allows extreme TIR angles and adjustments over a wide angle range. The penetration depth of the evanescent field (decrease to 1% intensity), which depends on the angle, can be reduced to about 150nm, rendering an exceptionally low depth of focus. Special highly refractive cover slips and immersion oil have to be employed to match the extreme NA. |
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UAPO 150xO TIRFM-SP (NA 1.45)
Unique high magnification TIRFM objective especially developed for single molecule applications. It features a compensation
collar for temperature and cover glass thickness. |
TIRFM Laser
| TIRFM Laser |
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Diode or diode-pumped solid state lasers
Five wavelengths available: 405 nm (50 mW), 488 nm (30 mW), 532 nm (20 or 50 mW), 561 nm (25 mW) and 635 nm (25 mW) |
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Polarisation-maintaining single mode fibre (except for 405 nm laser) |
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Directly mounted 405 nm laser head |
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20 levels of attenuation between about 0.2% and 100% |
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Fast shutter with 1 ms switching time |
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Diode or diode-pumped solid state lasers |
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Expected lifetime up to 10,000 h |
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Low operating noise |
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Compact housing |
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Control via hand switch, and / or external TTL trigger |
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Complying laser safety regulations |
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Class 3B laser product (IEC60825:1) |
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