Olympus - Your Vision Our Future

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The A to Z for Microscopy
B
Becke line
The Becke line appears as a band of light along the boundary between two media with different refractive indices. A Becke line might be observed between the mounting medium and crystal, or between adjacent crystals. It is caused by ...
Binning
Binning, also called on-chip integration, is the combination of the charges of neighboring CCD pixels during readout. The result is an increased signal and thus an improved sensitivity and a better signal-to-noise ratio. This allows to reduce the ...
Birefringence
Anisotropic crystals have crystallographically distinct axes and interact with light in a manner that is dependent upon the orientation of the crystalline lattice with respect to the incident light. When light enters a non-equivalent axis in ...
Bit Depth of Camera Data
Anisotropic crystals have crystallographically distinct axes and interact with light in a manner that is dependent upon the orientation of the crystalline lattice with respect to the incident light. When light enters a non-equivalent axis in ...
Bit Depth of Digital Images
The bit depth gives the number of possible greyscales (intensities) an image file format allows. For faster computer memory access, images are usually stored with a bit depth that is a multiple of 8. Black-and-white images taken with ...
Blooming
If a CCD is overexposed, that is, if the illumination exceeds the full-well capacity of the photodiodes in bright areas of the image, excess charges will overflow into adjacent wells and cause erroneously high intensities of the corresponding ...
Brightfield Observation
Brightfield illumination is probably the most widely used observation mode in optical microscopy. The technique is best suited for obervation of fixed, transparent and therefore stained specimens or other kinds of samples that naturally absorb ...