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The A to Z for Microscopy
F
FireWire
The IEEE1394, also called FireWire, is a modern plug-and-play interface between PC and ...
Fluorescence
The term fluorescence is derived from the mineral fluorspar (calcium fluoride). Fluorescence belongs to the ...
Fluorescence and Differential Interference Contrast (DIC) in combination
To minimise the effects of photobleaching, fluorescence microscopy can be combined with ...
Fluorescence and Phase Contrast in combination
To minimise the effects of photobleaching, fluorescence microscopy can be combined with phase contrast ...
Fluorescence Correlation Spectroscopy = FCS
Fluorescence correlation spectroscopy allows investigating molecular dynamics in highly diluted sample solutions. The researcher yields ...
Fluorescence in situ hybridisation = FISH
The term FISH refers to the common practice of fluorescence in situ hybridisation conducted on preparations of extended ...
Fluorescence Lifetime Imaging = FLIM
Multi-colour staining with fluorescent dyes is actively used for observing the distribution of biological materials (such as proteins, lipids, ...
Fluorescence microscopy system requirements
As explained under Limitations of fluorescence microscopy, the intensity of fluorescence is much lower than the light produced by the ...
Fluorescence microscopy, Limitations of
There are certain principal and practical limitations for fluorescence microscopy. Compared with the power of the excitation light, the observable fluorescence is ...
Fluorescence Photobleaching Investigations
Both fluorescence loss in photobleaching (FLIP) and the related methodology of recovery after photobleaching (FRAP) are techniques for ...
Fluorescent and Bioluminescent Proteins
If one is about to explain fluorescent and bioluminescent proteins the jellyfish Aequorea victoria is an outstanding example of an ...
Fluorescent probes
Many fluorescent dyes or fluorochromes can specifically interact with certain target molecules, like most nucleic acid stains (DAPI, ...
Fluorochromes
Fluorochromes, unlike classical tissue stains, re-radiate the absorbed light, however, with longer wavelength (red-shifted). Each fluorochrome has a ...
Foerster (Fluorescence-) Resonance Energy Transfer = FRET
The precise location and nature of the interactions between specific molecular species in living cells is of major interest in many areas of biological ...
Full-Well Capacity
This is the maximum amount of charges (electrons) the photodiodes of a CCD chip can accommodate. It is also called well depth and usually ...